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Proteintech rage
Rage, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rage/AGER+Antibody/pm42816162-105-7-9
Average 95 stars, based on 59 article reviews
rage - by Bioz Stars, 2026-10
95/100 stars

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Related Articles

Multiple Displacement Amplification:

Article Title: Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer’s Disease Induced by Amyloid Beta 1–42 Peptide
Article Snippet: .. Thymoquinone (Cat. No.: 490-91-5, Product Code: BTM-819813, Purity = 99%, Bostonchem USA Chemistry, 867 Boylston Street, Boston, MA, USA), Sesame oil (Cat. No.: HY-154629, Purity = 98.0%, MedChem Express, Monmouth Junction, NJ 08852, USA), Aβ 1–42 peptide (Cat. No.: HY-P1388, Purity = 98.28%, MedChem Express, Monmouth Junction, NJ 08852, Monmouth Junction, NJ 08852, USA), mouse MDA (Cat. No.: BLS-8658Mo, Bostonchem USA Chemistry, 867 Boylston Street, Boston, USA) kit, Anti-Aβ (Cat. No.: E-AB-70168, Elabscience, Houston, Monmouth Junction, NJ 08852, USA), Anti-RAGE (Cat. No.: 66833-1-Ig, Proteintech, Rosemont, IL 60018, USA), Anti-NOX4 (Cat. No.: 14347-1-AP, Proteintech, Rosemont, IL 60018, USA), citrate buffer (pH: 6.0; Thermo Fischer Scientific, Cambridge CB4 0GF, UK, AP-9003-500), Ultra V block solution (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-UB), biotinylated goat anti-polyvalent secondary antibody (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TP-125-BN), streptavidin peroxidase (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TS-125-HR), diaminobenzidine (DAB) chromogen (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-HD), Gill III Hematoxylin (Merck, Darmstadt 64293, Germany, 1.05174.1000), purezol (Cat. No.: 7326890, Bio-Rad, CA 94547, USA), cDNA Synthesis Kit (Cat. No.: SRK-1000, GenetBio, Daejeon, Republic of Korea). ..

Article Title: Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer's Disease Induced by Amyloid Beta 1-42 Peptide.
Article Snippet: .. Thymoquinone (Cat. No.: 490-91-5, Product Code: BTM-819813, Purity = 99%, Bostonchem USA Chemistry, 867 Boylston Street, Boston, MA, USA), Sesame oil (Cat. No.: HY-154629, Purity = 98.0%, MedChem Express, Monmouth Junction, NJ 08852, USA), Aβ1–42 peptide (Cat. No.: HY-P1388, Purity = 98.28%, MedChem Express, Monmouth Junction, NJ 08852, Monmouth Junction, NJ 08852, USA), mouse MDA (Cat. No.: BLS-8658Mo, Bostonchem USA Chemistry, 867 Boylston Street, Boston, USA) kit, Anti-Aβ (Cat. No.: E-AB-70168, Elabscience, Houston, Monmouth Junction, NJ 08852, USA), Anti-RAGE (Cat. No.: 66833-1-Ig, Proteintech, Rosemont, IL 60018, USA), Anti-NOX4 (Cat. No.: 14347-1-AP, Proteintech, Rosemont, IL 60018, USA), citrate buffer (pH: 6.0; Thermo Fischer Scientific, Cambridge CB4 0GF, UK, AP-9003-500), Ultra V block solution (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-UB), biotinylated goat anti-polyvalent secondary antibody (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TP-125-BN), streptavidin peroxidase (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TS-125-HR), diaminobenzidine (DAB) chromogen (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-HD), Gill III Hematoxylin (Merck, Darmstadt 64293, Germany, 1.05174.1000), purezol (Cat. No.: 7326890, Bio-Rad, CA 94547, USA), cDNA Synthesis Kit (Cat. No.: SRK-1000, GenetBio, Daejeon, Republic of Korea). ..

Blocking Assay:

Article Title: Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer’s Disease Induced by Amyloid Beta 1–42 Peptide
Article Snippet: .. Thymoquinone (Cat. No.: 490-91-5, Product Code: BTM-819813, Purity = 99%, Bostonchem USA Chemistry, 867 Boylston Street, Boston, MA, USA), Sesame oil (Cat. No.: HY-154629, Purity = 98.0%, MedChem Express, Monmouth Junction, NJ 08852, USA), Aβ 1–42 peptide (Cat. No.: HY-P1388, Purity = 98.28%, MedChem Express, Monmouth Junction, NJ 08852, Monmouth Junction, NJ 08852, USA), mouse MDA (Cat. No.: BLS-8658Mo, Bostonchem USA Chemistry, 867 Boylston Street, Boston, USA) kit, Anti-Aβ (Cat. No.: E-AB-70168, Elabscience, Houston, Monmouth Junction, NJ 08852, USA), Anti-RAGE (Cat. No.: 66833-1-Ig, Proteintech, Rosemont, IL 60018, USA), Anti-NOX4 (Cat. No.: 14347-1-AP, Proteintech, Rosemont, IL 60018, USA), citrate buffer (pH: 6.0; Thermo Fischer Scientific, Cambridge CB4 0GF, UK, AP-9003-500), Ultra V block solution (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-UB), biotinylated goat anti-polyvalent secondary antibody (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TP-125-BN), streptavidin peroxidase (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TS-125-HR), diaminobenzidine (DAB) chromogen (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-HD), Gill III Hematoxylin (Merck, Darmstadt 64293, Germany, 1.05174.1000), purezol (Cat. No.: 7326890, Bio-Rad, CA 94547, USA), cDNA Synthesis Kit (Cat. No.: SRK-1000, GenetBio, Daejeon, Republic of Korea). ..

Article Title: Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer's Disease Induced by Amyloid Beta 1-42 Peptide.
Article Snippet: .. Thymoquinone (Cat. No.: 490-91-5, Product Code: BTM-819813, Purity = 99%, Bostonchem USA Chemistry, 867 Boylston Street, Boston, MA, USA), Sesame oil (Cat. No.: HY-154629, Purity = 98.0%, MedChem Express, Monmouth Junction, NJ 08852, USA), Aβ1–42 peptide (Cat. No.: HY-P1388, Purity = 98.28%, MedChem Express, Monmouth Junction, NJ 08852, Monmouth Junction, NJ 08852, USA), mouse MDA (Cat. No.: BLS-8658Mo, Bostonchem USA Chemistry, 867 Boylston Street, Boston, USA) kit, Anti-Aβ (Cat. No.: E-AB-70168, Elabscience, Houston, Monmouth Junction, NJ 08852, USA), Anti-RAGE (Cat. No.: 66833-1-Ig, Proteintech, Rosemont, IL 60018, USA), Anti-NOX4 (Cat. No.: 14347-1-AP, Proteintech, Rosemont, IL 60018, USA), citrate buffer (pH: 6.0; Thermo Fischer Scientific, Cambridge CB4 0GF, UK, AP-9003-500), Ultra V block solution (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-UB), biotinylated goat anti-polyvalent secondary antibody (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TP-125-BN), streptavidin peroxidase (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TS-125-HR), diaminobenzidine (DAB) chromogen (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-HD), Gill III Hematoxylin (Merck, Darmstadt 64293, Germany, 1.05174.1000), purezol (Cat. No.: 7326890, Bio-Rad, CA 94547, USA), cDNA Synthesis Kit (Cat. No.: SRK-1000, GenetBio, Daejeon, Republic of Korea). ..

cDNA Synthesis:

Article Title: Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer’s Disease Induced by Amyloid Beta 1–42 Peptide
Article Snippet: .. Thymoquinone (Cat. No.: 490-91-5, Product Code: BTM-819813, Purity = 99%, Bostonchem USA Chemistry, 867 Boylston Street, Boston, MA, USA), Sesame oil (Cat. No.: HY-154629, Purity = 98.0%, MedChem Express, Monmouth Junction, NJ 08852, USA), Aβ 1–42 peptide (Cat. No.: HY-P1388, Purity = 98.28%, MedChem Express, Monmouth Junction, NJ 08852, Monmouth Junction, NJ 08852, USA), mouse MDA (Cat. No.: BLS-8658Mo, Bostonchem USA Chemistry, 867 Boylston Street, Boston, USA) kit, Anti-Aβ (Cat. No.: E-AB-70168, Elabscience, Houston, Monmouth Junction, NJ 08852, USA), Anti-RAGE (Cat. No.: 66833-1-Ig, Proteintech, Rosemont, IL 60018, USA), Anti-NOX4 (Cat. No.: 14347-1-AP, Proteintech, Rosemont, IL 60018, USA), citrate buffer (pH: 6.0; Thermo Fischer Scientific, Cambridge CB4 0GF, UK, AP-9003-500), Ultra V block solution (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-UB), biotinylated goat anti-polyvalent secondary antibody (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TP-125-BN), streptavidin peroxidase (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TS-125-HR), diaminobenzidine (DAB) chromogen (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-HD), Gill III Hematoxylin (Merck, Darmstadt 64293, Germany, 1.05174.1000), purezol (Cat. No.: 7326890, Bio-Rad, CA 94547, USA), cDNA Synthesis Kit (Cat. No.: SRK-1000, GenetBio, Daejeon, Republic of Korea). ..

Article Title: Evaluation of the Effects of Thymoquinone on RAGE/NOX4 Expressions and Brain Tissue Morphometry in Experimental Alzheimer's Disease Induced by Amyloid Beta 1-42 Peptide.
Article Snippet: .. Thymoquinone (Cat. No.: 490-91-5, Product Code: BTM-819813, Purity = 99%, Bostonchem USA Chemistry, 867 Boylston Street, Boston, MA, USA), Sesame oil (Cat. No.: HY-154629, Purity = 98.0%, MedChem Express, Monmouth Junction, NJ 08852, USA), Aβ1–42 peptide (Cat. No.: HY-P1388, Purity = 98.28%, MedChem Express, Monmouth Junction, NJ 08852, Monmouth Junction, NJ 08852, USA), mouse MDA (Cat. No.: BLS-8658Mo, Bostonchem USA Chemistry, 867 Boylston Street, Boston, USA) kit, Anti-Aβ (Cat. No.: E-AB-70168, Elabscience, Houston, Monmouth Junction, NJ 08852, USA), Anti-RAGE (Cat. No.: 66833-1-Ig, Proteintech, Rosemont, IL 60018, USA), Anti-NOX4 (Cat. No.: 14347-1-AP, Proteintech, Rosemont, IL 60018, USA), citrate buffer (pH: 6.0; Thermo Fischer Scientific, Cambridge CB4 0GF, UK, AP-9003-500), Ultra V block solution (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-UB), biotinylated goat anti-polyvalent secondary antibody (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TP-125-BN), streptavidin peroxidase (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TS-125-HR), diaminobenzidine (DAB) chromogen (Thermo Fischer Scientific, Cambridge CB4 0GF, UK, TA-125-HD), Gill III Hematoxylin (Merck, Darmstadt 64293, Germany, 1.05174.1000), purezol (Cat. No.: 7326890, Bio-Rad, CA 94547, USA), cDNA Synthesis Kit (Cat. No.: SRK-1000, GenetBio, Daejeon, Republic of Korea). ..

Incubation:

Article Title: Aβ impairs bone vascular homeostasis in APP/PS1 mice via disrupting the mitochondrial fission-efferocytosis axis in macrophages.
Article Snippet: Alzheimer’s disease (AD) is associated with progressive bone loss, but the underlying mechanisms remain unclear.. This study focused on how amyloid-β (Aβ) disrupted bone vascular homeostasis by impairing macrophage efferocytosis in APP/PS1 mice.. We found that Aβ accumulation in bone tissue impaired MerTK-mediated macrophage efferocytosis and promoted excessive accumulation of apoptotic endothelial cells (ECs).

Article Title: Neutrophil extracellular traps promote epithelial-mesenchymal transition in COPD via the RAGE/PI3K/AKT pathway.
Article Snippet: The concentration was measured using a BCA protein assay kit from Beyotime Biotechnology, China. .. Proteins were separated by SDS-PAGE with a concentration range of 6 %–10 %.Proteins were transferred to PVDF membranes, blocked with 5 % nonfat milk for 1 h, and incubated overnight at 4 ◦C with primary antibodies: anti-RAGE (1:1000, Proteintech, China), anti-p-PI3K p85 (1:1000, Thermo Fisher, USA), antiPI3K p85, anti-panAKT (1:1000, CST, USA), anti-pAKT (1:1000, CST, USA), anti-vimentin (1:1000, Invitrogen, USA), anti-E-cadherin (1:1000, Invitrogen, USA), and GAPDH as a control (1:10,000, Proteintech, China). .. This was followed by a 1-h incubation with HRP-labeled secondary antibodies at room temperature (1:2000, Proteintech, China).

Phospho-proteomics:

Article Title: Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice
Article Snippet: .. The primary antibodies, anti-PIGK (Cat#15151-1-AP), anti-EOGT (Cat#27595-1-AP), anti-RAGE (Cat#16346-1-AP), phosphorylation-p65 (Cat#82335-1-RR) and GAPDH (Cat#60004-1-Ig) were all purchased from Proteintech (Wuhan, China). .. Anti-TSPAN4(Cat#A10253) was purchased from Abclonal (Wuhan, China).

SDS Page:

Article Title: Neutrophil extracellular traps promote epithelial-mesenchymal transition in COPD via the RAGE/PI3K/AKT pathway.
Article Snippet: The concentration was measured using a BCA protein assay kit from Beyotime Biotechnology, China. .. Proteins were separated by SDS-PAGE with a concentration range of 6 %–10 %.Proteins were transferred to PVDF membranes, blocked with 5 % nonfat milk for 1 h, and incubated overnight at 4 ◦C with primary antibodies: anti-RAGE (1:1000, Proteintech, China), anti-p-PI3K p85 (1:1000, Thermo Fisher, USA), antiPI3K p85, anti-panAKT (1:1000, CST, USA), anti-pAKT (1:1000, CST, USA), anti-vimentin (1:1000, Invitrogen, USA), anti-E-cadherin (1:1000, Invitrogen, USA), and GAPDH as a control (1:10,000, Proteintech, China). .. This was followed by a 1-h incubation with HRP-labeled secondary antibodies at room temperature (1:2000, Proteintech, China).

Concentration Assay:

Article Title: Neutrophil extracellular traps promote epithelial-mesenchymal transition in COPD via the RAGE/PI3K/AKT pathway.
Article Snippet: The concentration was measured using a BCA protein assay kit from Beyotime Biotechnology, China. .. Proteins were separated by SDS-PAGE with a concentration range of 6 %–10 %.Proteins were transferred to PVDF membranes, blocked with 5 % nonfat milk for 1 h, and incubated overnight at 4 ◦C with primary antibodies: anti-RAGE (1:1000, Proteintech, China), anti-p-PI3K p85 (1:1000, Thermo Fisher, USA), antiPI3K p85, anti-panAKT (1:1000, CST, USA), anti-pAKT (1:1000, CST, USA), anti-vimentin (1:1000, Invitrogen, USA), anti-E-cadherin (1:1000, Invitrogen, USA), and GAPDH as a control (1:10,000, Proteintech, China). .. This was followed by a 1-h incubation with HRP-labeled secondary antibodies at room temperature (1:2000, Proteintech, China).

Control:

Article Title: Neutrophil extracellular traps promote epithelial-mesenchymal transition in COPD via the RAGE/PI3K/AKT pathway.
Article Snippet: The concentration was measured using a BCA protein assay kit from Beyotime Biotechnology, China. .. Proteins were separated by SDS-PAGE with a concentration range of 6 %–10 %.Proteins were transferred to PVDF membranes, blocked with 5 % nonfat milk for 1 h, and incubated overnight at 4 ◦C with primary antibodies: anti-RAGE (1:1000, Proteintech, China), anti-p-PI3K p85 (1:1000, Thermo Fisher, USA), antiPI3K p85, anti-panAKT (1:1000, CST, USA), anti-pAKT (1:1000, CST, USA), anti-vimentin (1:1000, Invitrogen, USA), anti-E-cadherin (1:1000, Invitrogen, USA), and GAPDH as a control (1:10,000, Proteintech, China). .. This was followed by a 1-h incubation with HRP-labeled secondary antibodies at room temperature (1:2000, Proteintech, China).



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Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell <t>marker</t> <t>Rage/Ager</t> in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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Image Search Results


Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell marker Rage/Ager in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Journal: bioRxiv

Article Title: Local IFNγ signaling contributes to the regenerative decline of aged alveolar progenitor cells

doi: 10.64898/2026.04.07.716929

Figure Lengend Snippet: Immunoproteasome is required for aged AT2 cell regenerative decline and the establishment of IFN γ+ T cells in tertiary lymphoid structures. A) Schematic figure to show the culture of alveolar organoids from young or aged immunoproteasome knockout (IP KO) and control C57BL/6 (WT) mice in feeder-free media at 7 days of growth, including B) organoid forming efficiency (OFE) and (C) diameter. D) RTqPCR of the IFNγ-inducible MHC-I complex component B2m in isolated lung AT2 cells from young or aged immunoproteasome KO and WT mice. E) Representative immunofluorescence staining of Ki-67 to mark proliferating cells with AT2 cell marker SPC and AT1 cell marker Rage/Ager in corresponding feeder-free organoids from aged mice in A-C. F) The ratio of Ki-67+ proliferating cells in aged immunoproteasome knockout (IP KO) versus WT mice. G) Dysregulated pathways in aged immunoproteasome KO epithelium in comparison to WT based on DEG genes (padj < 0.05, LogFC <0), with bar size representing enrichment score. H) Flow cytometry quantifications of CD8+ and CD4+ (I) T cells as % of all CD45+ immune cells in aged immunoproteasome KO and WT control lungs. J) Zoomed-in multiplex fluorescent images of TLS in aged lungs of immunoproteasome KO and WT controls with K) corresponding absolute quantification of CD8+ T cell and IFNγ+ cell (L) densities. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: We used the following primary antibodies: α-SPC (1:500, AB3786, Millipore), α-RAGE/AGER (1:500, MAB1179-100, R&D System), α-Ki-67 (1:200, 550609, BD Bioscience).

Techniques: Knock-Out, Control, Isolation, Immunofluorescence, Staining, Marker, Comparison, Flow Cytometry, Multiplex Assay, Quantitative Proteomics